Abstract The CJC-1295 with DAC 5mg peptide pen features a high-capacity, concentrated solution of the long-acting Growth Hormone-Releasing Hormone (GHRH) analogue. This molecule is distinguished by the addition of a Drug Affinity Complex (DAC)—specifically a maleimidopropionic acid linker—to the lysine residue at the C-terminus. This modification enables the peptide to form a covalent bond with serum albumin in vivo, shielding it from DPP-IV enzymatic degradation and renal filtration. The result is an extended half-life of approximately 6-8 days. This 5mg formulation is specifically designed for longitudinal studies requiring sustained, basal elevation of Growth Hormone (GH) and Insulin-Like Growth Factor 1 (IGF-1), offering a high-volume reagent suitable for larger animal models or extended duration protocols.

Mechanism and Research Interest

Primary Biological Pathway: Albumin Bioconjugation and Stability The defining mechanism of CJC-1295 with DAC is its pharmacokinetics. Upon subcutaneous administration, the reactive maleimide group binds to Cys34 on circulating albumin. This bioconjugation transforms the small peptide into a large protein complex, effectively removing it from the rapid clearance pathways that limit native GHRH (which has a half-life of minutes). Research utilising the 5mg pen allows for the study of “GH bleed,” maintaining tonic activation of the pituitary somatotrophs to drive continuous, rather than pulsatile, GH secretion.

Secondary Research Finding: Maximal IGF-1 Production While pulsatile GH release is physiological, continuous GHRH stimulation is far more effective at driving hepatic IGF-1 production. The 5mg formulation is the standard reagent for models of severe muscle wasting or catabolism where the research goal is to maximise the “Area Under the Curve” (AUC) of circulating IGF-1. Studies utilizing this high-concentration format often document significant increases in protein synthesis rates and nitrogen retention compared to pulsatile controls.

Tertiary Research Finding: Lipolysis and Body Composition Continuous GH elevation exerts a potent lipolytic effect. It increases the expression of $\beta$-adrenergic receptors on adipocytes and inhibits lipoprotein lipase (LPL). Researchers use the 5mg pen in obesity models to investigate the “fat-stripping” phenotype associated with acromegaly-like hormonal profiles, specifically measuring the reduction in visceral adipose tissue mass over multi-week protocols.

Long-term Genomic and Safety Observations The 5mg pen is particularly useful for safety pharmacology studies investigating Tachyphylaxis. There is a theoretical concern that constant stimulation could desensitise pituitary receptors or deplete GH stores. High-load studies (enabled by the 5mg capacity) monitor Ghrhr mRNA expression and pituitary volume to determine if the gland adapts to chronic stimulation by downregulating sensitivity or expanding capacity (hyperplasia).

Product Specifications

• Purity: Validated at >98+% via High-Performance Liquid Chromatography (HPLC). We rigorously test the DAC moiety to ensure it remains reactive for albumin binding but has not pre-reacted during synthesis or storage.

• Appearance: The 3ml pen contains a clear, colourless, sterile liquid.

• Precision: The pen delivery system ensures accurate volumetric dosing. The 5mg concentration allows for the administration of higher weekly doses (e.g., 1mg – 2mg) required for primate or large canine models without the need for multiple injections.

• Storage: The product must be stored at 2∘C to 8∘C.

Storage and Shipping Guidelines

Refrigeration and Shelf Life The CJC-1295 with DAC 5mg Pen contains a reactive peptide species. Refrigeration (2∘C to 8∘C) is mandatory. The shelf life is 12 months in the sealed state. Once the pen is accessed, the bacteriostatic environment preserves the solution for 28 days. The pH of the solution is carefully buffered to prevent the maleimide group from hydrolysing into a non-reactive form before administration.

Shipping Stability We utilise medical-grade thermal packaging. The peptide is stable for up to 72 hours at ambient UK temperatures. However, to preserve the bioconjugation efficiency essential for its long half-life, we strongly recommend selecting the fastest available shipping option.

Freezing Warning Do not freeze. Freezing can precipitate the peptide and permanently deactivate the DAC linker. A frozen pen will likely function only as a short-acting GHRH analogue (if at all), completely altering the pharmacokinetic profile of your experiment. A frozen pen should be discarded.

Why Choose the Peptide Pro 3ml Pen Format?

The CJC-1295 with DAC 5mg Pen is the definitive tool for High-Load and Longitudinal Research. While the 2mg pen is suitable for small rodent studies, the 5mg format is essential for researchers working with larger models or running extended protocols where consistent batch usage is critical to minimise variability.

It provides a verified, high-concentration source of the long-acting analogue, eliminating the confusion with “No DAC” variants and removing the risks associated with solubilising reactive peptide complexes in non-optimised buffers.

For researchers requiring pulsatile (physiological) stimulation, we recommend examining:

• CJC-1295 No DAC 10mg: (For short-acting, synergistic research that mimics natural rhythms).

• Ipamorelin 10mg: (For selective, non-stressful pulse generation).

Disclaimer

This product is strictly for Research Use Only (RUO). It is not intended for human consumption, injection (e.g., for bodybuilding, anti-ageing, or performance enhancement), therapeutic use, or diagnostic procedures. The information provided is for educational and scientific reference only. Purchase is restricted to verified research institutions and qualified individuals. Any evidence of intended misuse for human application will result in immediate order cancellation and blacklisting, in compliance with UK research chemical regulations.